problems with primer dimer!

Stefi~ xxxgifalone at tin.it
Mon Aug 27 13:18:43 EST 2001


Babett Bartling <babett.bartling at medizin.uni-halle.de> wrote in message
3B8A4C6B.3DEC2735 at medizin.uni-halle.de...
> Hope somebody can help me.
> We investigated gene expression using PCR without any problems.
> Since last week, we suddenly have a lot of primer dimers and problems
> with the right size band.
> How can we solve the problem with the excess of primer dimers??

try to denaturate primer dimers with 94°C for 5-10 minutes. If it doesn't
work, order new primers and aliquot them in 10uL or so and put them
at -20°C. It's the cheapest way.
Stefano
xxxgifalone at tin.it
(rimuovere xxx per rispondere privatamente)

http://web.tiscalinet.it/gifalone
ICQ 110419911




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