DNA methylation question

Robert Whittier rfwhittier at hotmail.com
Tue Aug 28 21:31:09 EST 2001


>Could you noble experts plase help me out?

Schmeichlerei will get you everywhere!

>
>What doest it exacly mean when a restriction enzyme is blocked by 
>OVERLAPPING Dam methylation?
>
>Do I understand the phenomenon correctly in that example: XbaI (TCTAGA) 
>does not cut the sequence GATCtaga since there will be methylation on the 
>reverse strand at the position of the T (then an A) of GATC, yielding 
>TCTAGAm on the reverse strand? (when using any standard E. coli strain for 
>propagation of DNA)

Absolutely correct.

/Bob

Tokyo


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