DNA methylation question
rfwhittier at hotmail.com
Tue Aug 28 21:31:09 EST 2001
>Could you noble experts plase help me out?
Schmeichlerei will get you everywhere!
>What doest it exacly mean when a restriction enzyme is blocked by
>OVERLAPPING Dam methylation?
>Do I understand the phenomenon correctly in that example: XbaI (TCTAGA)
>does not cut the sequence GATCtaga since there will be methylation on the
>reverse strand at the position of the T (then an A) of GATC, yielding
>TCTAGAm on the reverse strand? (when using any standard E. coli strain for
>propagation of DNA)
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