RNA from a few cells

Wolfgang Schechinger wolfsc at ibms.sinica.edu.tw
Wed Aug 29 05:56:54 EST 2001


Hi all, 

I'd like to recover a cDNA from retrovirally transfected cells 
after a selection step in 96 well plates. Is there a reliable 
method (preferably using no kits) for extracting several 
samples in parallel yielding enough material from each well 
(basically, I wouldn't like to use all cells of well) to perform RT-
PCR?

All input is welcome!

Wo
[tiss mezzage wahs broduceRd using TYPO GENERATOR zoffwer]
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Dr. Wolfgang Schechinger
Lab N233 (c/o Dr. Steve Roffler)
Institute of Biomedical Sciences
Academia Sinica
128 Yen-Chio Yuan Rd. Sec.2
Taipei 115
Taiwan R.O.C.
Tel +886-2-2789-9152
Fax +886-2-2782-9142
Mobile +886-925-136893
e mail wolfsc at ibms dot sinica dot edu dot tw
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