Add: RNA from a few cells

[Wolfgang Schechinger]

Something to add:

Or is there maybe some magic RT-PCR directly starting with 
a few cells (Balb/3T3) in the eppi? 

Wo


> Hi all, 
> 
> I'd like to recover a cDNA from retrovirally transfected cells
> after a selection step in 96 well plates. Is there a reliable
> method (preferably using no kits) for extracting several samples
> in parallel yielding enough material from each well (basically, I
> wouldn't like to use all cells of well) to perform RT- PCR?
> 
> All input is welcome!
> 
> Wo
> [tiss mezzage wahs broduceRd using TYPO GENERATOR zoffwer]
> -----
> Dr. Wolfgang Schechinger
> Lab N233 (c/o Dr. Steve Roffler)
> Institute of Biomedical Sciences
> Academia Sinica
> 128 Yen-Chio Yuan Rd. Sec.2
> Taipei 115
> Taiwan R.O.C.
> Tel +886-2-2789-9152
> Fax +886-2-2782-9142
> Mobile +886-925-136893
> e mail wolfsc at ibms dot sinica dot edu dot tw
> -----
> 
> ---
> 
> 


[tiss mezzage wahs broduceRd using TYPO GENERATOR zoffwer]
-----
Dr. Wolfgang Schechinger
Lab N233 (c/o Dr. Steve Roffler)
Institute of Biomedical Sciences
Academia Sinica
128 Yen-Chio Yuan Rd. Sec.2
Taipei 115
Taiwan R.O.C.
Tel +886-2-2789-9152
Fax +886-2-2782-9142
Mobile +886-925-136893
e mail wolfsc at ibms dot sinica dot edu dot tw
-----

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