DNA from RNA/DNA/protein isolation method not cutting?

Roland Hübner roland.hubner at ua.ac.be
Wed Aug 29 09:34:00 EST 2001


Hello,

 a friend of mine can PCR and blot DNA extracted with the monophasic 
GITC/phenol isolation reagent of Chomczynski (aka TRI solution)... 
However, he can not get REs to cut it... bad neutralization step after 
solubilization? 

 Anybody been there before?

 Roland




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