Recombination in E. coli

Michael Witty mw132 at mole.bio.cam.ac.uk
Thu Aug 30 09:43:19 EST 2001


Dear Wolfgang,
             it would have to be recombination that put the glucuronidase
gene into the chromosome, but not the plasmid ori (this is detrimental to
the bacterium isn't it?).  You could check with PCR couldn't you?  Mike.

On 30 Aug 2001, Wolfgang Schechinger wrote:

> Hi all,
>
> Just a question: When I transfect E. coli (HB 101) with a
> vector that contains an E. coli gene (cDNA for beta
> glucuronidase), could it be that recombination occurs?
>
> I did a maxiprep but couldn't find any visible precipitate in the
> eluate. The bugs were growing in LB-Amp...
>
> All input is welcome
> Wo
> [tiss mezzage wahs broduceRd using TYPO GENERATOR zoffwer]
> -----
> Dr. Wolfgang Schechinger
> Lab N233 (c/o Dr. Steve Roffler)
> Institute of Biomedical Sciences
> Academia Sinica
> 128 Yen-Chio Yuan Rd. Sec.2
> Taipei 115
> Taiwan R.O.C.
> Tel +886-2-2789-9152
> Fax +886-2-2782-9142
> Mobile +886-925-136893
> e mail wolfsc at ibms dot sinica dot edu dot tw
> -----
>
> ---
>




More information about the Methods mailing list