Recombination in E. coli
sergioal at bbm1.ucm.es
Fri Aug 31 07:01:12 EST 2001
Wolfgang Schechinger wrote:
> Hi all,
> Just a question: When I transfect E. coli (HB 101) with a
> vector that contains an E. coli gene (cDNA for beta
> glucuronidase), could it be that recombination occurs?
> I did a maxiprep but couldn't find any visible precipitate in the
> eluate. The bugs were growing in LB-Amp...
> All input is welcome
Maybe too simple, but it happened once in our lab (we got no plasmid from
"supposed to be recombinant" cells): check the antibiotic.
More information about the Methods