Recombination in E. coli

Sergio sergioal at bbm1.ucm.es
Fri Aug 31 07:01:12 EST 2001



Wolfgang Schechinger wrote:

> Hi all,
>
> Just a question: When I transfect E. coli (HB 101) with a
> vector that contains an E. coli gene (cDNA for beta
> glucuronidase), could it be that recombination occurs?

>
> I did a maxiprep but couldn't find any visible precipitate in the
> eluate. The bugs were growing in LB-Amp...
>
> All input is welcome
>

Maybe too simple, but it happened once in our lab (we got no plasmid from
"supposed to be recombinant" cells): check the antibiotic.

Sergio





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