Recombination in E. coli

Sergio sergioal at
Fri Aug 31 07:01:12 EST 2001

Wolfgang Schechinger wrote:

> Hi all,
> Just a question: When I transfect E. coli (HB 101) with a
> vector that contains an E. coli gene (cDNA for beta
> glucuronidase), could it be that recombination occurs?

> I did a maxiprep but couldn't find any visible precipitate in the
> eluate. The bugs were growing in LB-Amp...
> All input is welcome

Maybe too simple, but it happened once in our lab (we got no plasmid from
"supposed to be recombinant" cells): check the antibiotic.


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