SDS PAGE

Tyson mediablitz at home.com
Mon Dec 3 14:39:08 EST 2001


what about your samples? are they running ok?  Is your BPB marker running
ok? If so than your protein marker must be the problem...try some other
markers or get a new one.

"Nadim" <nms68 at columbia.edu> wrote in message
news:20011203034902.10226.qmail at ww02.jatek.com...
> Hi
>
> I have been having problems with running my SDS PAGE. Ive bee running an
8%
> running with a 4% stacking yet recently for some baffling reason my marker
> has not been seperating correctly. I am only seeing the top two (heaviest
> two) bands appear immediately once the samples get to the running part.
Then
> only the heaviest two bands (120, and 90KD) are migrating throughout the
> gel, it is almost as if the remaining bands of the marker are 'too small'
> for the gel and dont appear! Ive made new Tris buffers and even though Im
> using the same acrylamide others in the lab have used it with no problems.
> Ive also made fresh running buffer. I checked my recipes and they are
> correct, yet i still see the smae phenomenon. I am baffled.
>
>
> <http://www.biowww.net/forum/read.php?f=1&i=4806&t=4806>
>





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