Fungal identification by PCR

Duncan Clark junk at 127.0.0.1
Thu Dec 6 04:01:49 EST 2001


In message <20011205225740.17972.qmail at ww02.jatek.com>, sasho 
<sasho at mt.net.mk> writes on the Wed, 5 Dec 2001
>We have isolated DNA and successfully amplified a region
>from fungal rRNA gene by ITS-1 and ITS-4 primer set and found slightly
>different size of amplicons ranging app. 500 to 650 bp.
>Please help me to design an experiment to identify the fungal genus, family
>or species by PCR or subsequently by RFLP or sequencing.

Why not isolate, re-amplify and sequence each individual PCR product?

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....

Duncan Clark
GeneSys Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382




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