pcr from glycerol stock!

Sushma Tiwari bsssmt at bath.ac.uk
Thu Dec 6 08:05:23 EST 2001


Hi,
I would like to know if any of you have done PCR directly from a culture
in glycerol stock. Is it possible, If yes could you share your
protocols/experiences with me please.
I have to amplify clones which are in 384 well format (1500 colonies)
and they are in glycerol stocks, I would have to spend a lot of money
and energy if i have to grow them individually and padmid prep all of
them.
Any ideas will be welcome,

sumis




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