Baculovirus transfer vectors, Compatibility between brands ??

Svend Kjaer svend at cajal.mbb.ki.se
Thu Dec 6 10:08:49 EST 2001


Dear all,

I am about to express MFP in a baculovirus system. The transfer vector
system is based on the pVL1393 from Pharmigen. As it happens, our lab
has a stock of transfection material from Clontech, who uses the pBacPAK
series of vectors as transfer vectors.
My question is therefore very simple, is the transfection mix the same
in both cases or do you need to buy the Pharmigen "baculogold" kit to
get the virus ??

Thanks in advance for any input,

Svend

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Svend Kjær, Ph.D.
Division of Molecular Neurobiology,
Department of Neuroscience
Karolinska Institute
Retzius väg 8, A2:2
171 77 Stockholm
Sweden

Tel: +46-8-7287666
Fax: +46-8-339548

Private phone: +46-8-6409962
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