nonisotopic RNA detection

And'ys bionet andrew.bettany at bbsrc.ac.uk
Fri Dec 7 03:33:32 EST 2001


I think I would echo what has been said. We use the DIG system routinely for
genomic Southerns, but are really struggling to get Northerns to work. I
would warn you not to expect it to work without some "tinkering"!


--
Dr. Andy Bettany
Research Scientist
Institute of Grassland and Environmental Research
Plas Gogerddan
Wales, UK

"AvV" <avvliet at ision.nl> wrote in message
news:1007680227.559934 at news.knoware.nl...
> I use the DIG system successfully with bacterial RNA, and it works (most
of
> the time). Took a lot of time to set up, but bacterial RNA is much more
> difficult than most eukaryotic RNA work. Most important in my opinion is
to
> use RNA probes prepared by in vitro transcription, independent of the
> labeling method used.
>
> Arnoud
>
> "R. John Lye" <rjl6n at virginia.edu> wrote in message
> news:3C0F63C7.F222A2B at virginia.edu...
> > Cindy Graham wrote:
> >
> > >  I am thinking of using Ambion's bright-star system.
> >
> > I've used the Bright-Star system for Southerns and loved it.  I haven't
> > tried Northerns with it yet, though.
> >
> > John
> >
> >
>
>





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