Question about quantitating western blots.

Michael L. Sullivan mlsulliv at facstaff.wisc.edu
Fri Dec 7 09:26:40 EST 2001


Absolutely, DK!  Western blots have got to be one of the most non-linear
systems I've ever worked with!

Mike

>
>The best way to quantitate Westerns is to include calibration curve
>samples in the same blot. The worst way is not to do it and pretend that
>that pixels are linearly proportional to protein. The detection method
>is really secondary and determines mostly sensitivity range you are
>after.
>
>DK


Michael L. Sullivan, Ph.D

U.S. Dairy Forage Research Center
1925 Linden Drive West
Madison WI, 53706

(608) 264-5144 Phone
(608) 264-5147 Fax

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