Optimal cDNA length in expression vectors?
wolfsc at ibms.sinica.edu.tw
Sat Dec 8 03:45:36 EST 2001
in my experince with pCDNA3 (and also in general), just using the sequence
from start to stop works fine. If you add 5' UTR, you'll incerease the
distance to the CMV promoter which is upstream and maybe insert some undesired
Adding a Kozak consensus element around the ATG (eg gccgccaccATGg) could
> I have a basic question regarding expression vectors.
> I would like to clone cDNA's into an expression vector (e.g. pcDNA3) in
> order to do transfection experiments. Clearly, in order to get
> functional protein, the inserted cDNA should contain the whole sequence
> from start (ATG) codon to stop codon. But how does the length of any
> included 5'UTR (or 3'UTR) influence optimal transgene expression? When
> I clone a gene of interest into the MCS of a commercial available
> expression vector, is it ok to start directly with ATG?
> Thanks a lot for any comments
Dr. Wolfgang Schechinger
Institute of Biomedical Sciences
Academia Sinica, Taipei, Taiwan R.O.C.
e mail wolfsc at ibms dot sinica dot edu dot tw
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