Transfection by Ca-phosphate of a B-cell line
Richard P. Grant
rpg14 at yahoo.co.uk.invalid
Mon Dec 10 10:08:22 EST 2001
In article <1c8875d6.0112100700.1bc3433f at posting.google.com>,
ruben.varela-calvino at kcl.ac.uk (Ruben Calvino) wrote:
> Any suggestion? What is going on here? I just tried to spin any
Essentially you're pouring concrete on your cells ;) My advice would be
to try one of the lipo reagents, such as Lipofectamine or
Transfectamine. Fairly straightforward, and a lot nicer to your cells.
Tend to get higher rates of transfection, too. The only thing to watch
is that you may have to perform the t/fection in SF media.
That's as may be, but RPG is also a Scientist, and, as with dragons, it
isn't wise to provoke scientists, for you are crunchy and would make an
excellent vector for new pathogens.
- CK in csmgf-s
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