growing cells under agarose
r.hartley at bio.gla.ac.uk
Tue Dec 11 04:47:50 EST 2001
""Wolfgang Schechinger"" <wolfsc at ibms.sinica.edu.tw> wrote in message
news:3C163481.23664.DF4C2E4 at localhost...
> hi all,
Hi Wolfgang I'm back.
> I'd like to cover fibroblasts with agarose or another protein free jelly.
> Is there anyone aware of how to pour liquid agarose (or equivalent) onto
> the cells without boiling them?
is this what you mean?
Seems the agarose solidifies (guess) about 42-45C so heat the cells upa bit
to prevent heatshock or simply pour on.
> Your input is appreciated!
Douglas A. Lauffenburger hs some papers on agarose you might find some stuff
Bob; Sunny Scotland
> Dr. Wolfgang Schechinger
> Institute of Biomedical Sciences
> Academia Sinica, Taipei, Taiwan R.O.C.
> e mail wolfsc at ibms dot sinica dot edu dot tw
More information about the Methods