growing cells under agarose

bob r.hartley at bio.gla.ac.uk
Tue Dec 11 04:47:50 EST 2001


""Wolfgang Schechinger"" <wolfsc at ibms.sinica.edu.tw> wrote in message
news:3C163481.23664.DF4C2E4 at localhost...
> hi all,

Hi Wolfgang I'm back.
>
> I'd like to cover fibroblasts with agarose or another protein free jelly.
> Is there anyone aware of how to pour liquid agarose (or equivalent) onto
> the cells without boiling them?

see
www.cb.uu.se/verksamhet/annual_report/AR00html/node28.html
is this what you mean?

Seems the agarose solidifies (guess) about 42-45C so heat the cells upa bit
to prevent heatshock or simply pour on.
>
> Your input is appreciated!

Douglas A. Lauffenburger hs some papers on agarose you might find some stuff
there.

cheers
Bob; Sunny Scotland
>
> Regards,
> Wo
> -----
> Dr. Wolfgang Schechinger
> Institute of Biomedical Sciences
> Academia Sinica, Taipei, Taiwan R.O.C.
> e mail wolfsc at ibms dot sinica dot edu dot tw
> -----
>
> ---




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