growing cells under agarose

Sergio sergioal at bbm1.ucm.es
Tue Dec 11 05:38:21 EST 2001



Wolfgang Schechinger ha escrito:

> hi all,
>
> I'd like to cover fibroblasts with agarose or another protein free jelly.
> Is there anyone aware of how to pour liquid agarose (or equivalent) onto
> the cells without boiling them?
>
> Your input is appreciated!
>
> Regards,
> Wo

I never tried on eukariotic cells, but maybe you could use low gel temperature
agarose (it stays liquid at 40ºC and forms the gel around 37ºC). Once it has
formed the gel, it supports more than 65ºC.

Sergio




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