PCR contamination

Duncan Clark junk at 127.0.0.1
Tue Dec 11 08:17:45 EST 2001


In message <3C154379.ACC424CC at ucalgary.ca>, Neal <nrmelvin at ucalgary.ca> 
writes on the Mon, 10 Dec 2001
>I am not doing an RNAse step at
>all... could this be a contaminating RNA (ribosomal) species?

So is it there in the PCR before you start the cycling? If so then it is 
possible. What happens if you add RNase?

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....

Duncan Clark
GeneSys Ltd.




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