growing cells under agarose

Stewart P Johnson Johns053 at mc.duke.edu
Tue Dec 11 08:40:37 EST 2001


You could try using soft agar as per the soft agar cloning procedure except
instead of mixing your cells with the agarose, pour the agarose over the
fibroblasts.  In that instance, the Seaprep low melting temp agarose is
cooled to 37 C before adding the cells.  After pouring the agarose, cool
the plates at 4 C until the agarose gels and then place them in the
incubator.  Good luck, Stewart




wolfsc at ibms.sinica.edu.tw ("Wolfgang Schechinger")@hgmp.mrc.ac.uk on
12/11/2001 03:24:55 AM

Sent by:  owner-methods at hgmp.mrc.ac.uk


To:   methods at hgmp.mrc.ac.uk
cc:
Subject:  growing cells under agarose


hi all,

I'd like to cover fibroblasts with agarose or another protein free jelly.
Is there anyone aware of how to pour liquid agarose (or equivalent) onto
the cells without boiling them?

Your input is appreciated!

Regards,
Wo
-----
Dr. Wolfgang Schechinger
Institute of Biomedical Sciences
Academia Sinica, Taipei, Taiwan R.O.C.
e mail wolfsc at ibms dot sinica dot edu dot tw
-----

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