16S sequencing primers

Wolfgang Schechinger wolfsc at ibms.sinica.edu.tw
Tue Dec 11 11:44:08 EST 2001


Dear Sergio

Google finds everything :-((( (!!)

http://ww.google.com/search?q=Lane+16S+23S+rRNA+sequencing+primer

Regards, 
Wo



> Hi all,
> i've sequenced the 16S rRNA from a common bacteria, using some primers which i
> only know the name, not the sequence. I've been told their sequences are publish
> in the following book:
> 
> Lane,D.J. (1991). "16S/23S rRNA sequencing." In: E. Stackebrandt and M.
> Goodfellow (Eds.), Nucleic acid
>      techniques in bacterial systematics, John Wiley and Sons, New York, NY, pp.
> 115-175.
> 
> but i cannot find it in my center. May somebody send me the sequence of the
> following primers:
> 
> 27f ,437f, 1074f, 1100r, 519r, 785r, 806f and 1492r
> 
> or point me to a web site i can get the sequences from (or a paper reference).
> 
> Thanks in advance
> 
> 


-----
Dr. Wolfgang Schechinger
Institute of Biomedical Sciences
Academia Sinica, Taipei, Taiwan R.o.C.

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