PCR contamination

Stefi~ xxxgifalone at tin.it
Tue Dec 11 12:42:18 EST 2001


"Neal" <nrmelvin at ucalgary.ca> ha scritto nel messaggio
news:3C154379.ACC424CC at ucalgary.ca...
> I seem to be getting an 1100bp band on my SybrGold stained gels after
> genomic PCR... this appears regardless of the primers I've used, but
> never appears in my no template control. I am not doing an RNAse step at
> all... could this be a contaminating RNA (ribosomal) species?

I don't think so. Maybe you have an incorrect annealing of your primer(s).
Anyway, try to digest your sample with RNase.
Let us know.
Stefano
xxxgifalone at tin.it
(remove antispam xxx)




More information about the Methods mailing list