protein solubility

shan hshandilya at yahoo.co.in
Thu Dec 13 13:00:44 EST 2001


hi.

I have a problem regarding the solubility of my protein. This protein has a
cellulose binding domain tag. the size of the proein is almost 35kDa. This
protein has huge expression but it is going in the inclusion body in
insoluble form. I tried to solubilize it by 6M urea it became solubalised
but when I dialysed against decrease urea gradient it again became
insoluble.  Some one has the idea how to come out of this problem. any
suggestion will be appericiated

shan


<http://www.biowww.net/forum/read.php?f=1&i=4883&t=4883>




More information about the Methods mailing list