protein solubility

[shan]

hi.

I have a problem regarding the solubility of my protein. This protein has a
cellulose binding domain tag. the size of the proein is almost 35kDa. This
protein has huge expression but it is going in the inclusion body in
insoluble form. I tried to solubilize it by 6M urea it became solubalised
but when I dialysed against decrease urea gradient it again became
insoluble.  Some one has the idea how to come out of this problem. any
suggestion will be appericiated

shan


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