immuno-ppt/Western intensities --> expression?

Roland Hübner roland.hubner at ua.ac.be
Thu Dec 13 16:05:14 EST 2001


Hello,

 I have the following questions:

 Alternative splicing yielding 2 proteins will not necessarily produce 
the same amount of both forms... If antibodies are used to recognize 
both forms after immuno-ppt. and/or Western & one of the forms is 
strongly stained whereas the other is very weakly stained, can this be 
used as a measure of expression of each form? Furthermore, can this 
observed result be used to predict the outcome of IHC staining with same 
antibodies?

 Thanks for any examples, refs, supporting/disclaiming data, ...

Best wishes,
 Roland




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