immuno-ppt/Western intensities --> expression?

Nick Theodorakis nicholas_theodorakis at urmc.rochester.edu
Thu Dec 13 17:10:24 EST 2001


In <3c19180a$1 at news.uia.ac.be>, Roland Hübner wrote:
>
>Hello,
>
> I have the following questions:
>
> Alternative splicing yielding 2 proteins will not necessarily produce 
>the same amount of both forms... If antibodies are used to recognize 
>both forms after immuno-ppt. and/or Western & one of the forms is 
>strongly stained whereas the other is very weakly stained, can this be 
>used as a measure of expression of each form? Furthermore, can this 
>observed result be used to predict the outcome of IHC staining with 
same 
>antibodies?
>

I think it would be likely true if it was a monoclonal or monospecific Ab that 
recognized an epitope present on both proteins, assuming that the 
epitope was found the same number of times (e.g., once) on both 
proteins. No reference, just a strong hunch.

Nick

-- 
Nick Theodorakis
nicholas_theodorakis at urmc.rochester.edu

 




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