DNA gels-separation of 100 bp between 1000 and 1500 bp

Michael Witty mw132 at mole.bio.cam.ac.uk
Thu Dec 13 20:25:04 EST 2001

On 13 Dec 2001 vuvuvu1 at supereva.it wrote:

> Hi
> I'd appreciate your help with the following:
> I have to separate the range between 1000 and 1500 bases and see small
> differences (like evry 50 bases) in between
> I tried 0.9 to 2 % agarose over 16 cm  (0.9 letting BB out) but I can't
> get the separation I need, Ultimately I'd like to find a condition where
> 1500 bases stays on top of the gel and 1000 on the bottom
> I wonder if  I should  do a denaturing polyacrilamide gel instead but
> can't find a table with the appropriate percentage to use
> in non denaturing gels migration is also a function of base composition,
> is it the same in agarose gels?
> thanks
> Vuvu

Dear Vuvu,
          I think Maniatis edition 1 has a method for DNA PAGE.  They are
not denaturing and they are very good for tens to hundreds of bp.  Mike.

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