EtBr (is healthy!)
wolfsc at ibms.sinica.edu.tw
Fri Dec 14 00:09:54 EST 2001
to put an end to the re-occurring discussion, please read that.
(Everything downstream is citation from US National Toxicology Program)
" REAGENT OF DNA CHEMISTRY; DNA INTERCHELATING AGENT FOR
BIOCHEMICAL & MOLECULAR BIOLOGY RESEARCH. SOME REPORTED
USE AS AN ANTIPARASITIC & ANTIPROTAZOIC DRUG IN ANIMALS; HAS
ALSO BEEN USED AS A DRUG OR DRUG PRECURSOR. "
"Animal Data: No 2-year bioassay studies associating EB either positively or
negatively with a carcinogenic effect in animals were found in the available
"There is some suggestion in references such as catalog cited that above EB
may be carcinogenic in animals, but they appear not to be substantiated by
adequate studies but rather to reflect inference based on mutagenic and DNA-
damaging activity. [N.B. Fritzenschaf et al. (1993) reported that EB was
determined to be carcinogenic in vivo in a 1970 study attributed to Boller, K.
and Schmid, W. and published in Humangenetik, 11:35-54: attempts to locate and
retrieve this citation were unsuccessful.] On the other hand, several early
'70s studies reported that EB demonstrated antitumorigenic effects. Balda and
Birkmayer (1973) reported EB to be potentially therapeutic based on specific
action against hamster melanoma A Mel 3 cells (a model for human malignant
melanoma) which prolonged survival time in experimental animals. The mechanism
of action was thought to be based on strong inhibition of RNA-directed DNA
Nishiwaki et al. (1974) also reported EB to be antitumorigenic based on
inhibition of RNA-dependent DNA polymerase activity in experimental tumor
systems. They attributed increased life spans in mice with 6C3HED-OG (200%)
and mice with L5178Y (83%) to the antileukemic activity of EB."
Note: You cannot imagine what EtBr area looks like here. You enter it like
Dr. Wolfgang Schechinger
Institute of Biomedical Sciences
Academia Sinica, Taipei, Taiwan R.o.C.
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