please tell me the sequence of vector pFL61

=?gb2312?q?shude=20yang?= sdyang68 at yahoo.com.cn
Fri Dec 14 02:05:28 EST 2001


Hello,Everyone:

Who can tell me where I can find the the sequence of
pFL61,a yeast vector .I have searched for many times
on the web,but failed. Since BstXI site can only
eliminates vector self-ligation ,but can not
facilitates directional cloning of foreign cDNA,so I
want to reconstructe pFL61,I want to introduce
asymmetrical SfiI sites. Can you give me some
practical advice? Any information about vector
construction is also welcomed.Thank you!

With my best wishes
sdyang

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