xho1 ligation problem

Richard P. Grant rpg14 at yahoo.co.uk.invalid
Sat Dec 15 03:25:17 EST 2001

In article <4.1.20011214164526.009d27a0 at students.wisc.edu>,
 alprasch at students.wisc.edu (Amy Prasch) wrote:

> I am trying to make a construct and am stuck on this step. I want to cut my
> insert out of the donor vector using xho1, linearize my acceptor vector
> using xho1 ( and CIAP treat) 

Are you deactivating the CIAP (phenol-chloroform)?  I'd recommend the 
Shrimp Alkaline Phosphatase; better enzyme, heat inactivable.

Richard P. Grant  0x5F9559B1                         MRC Lab of Mol Biol
rpg 'at' mrc-lmb.cam.ac.uk   http://www2.mrc-lmb.cam.ac.uk/personal/rpg/
       'Practising biochemistry without a licence' - E. Chargaff

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