protein solubility

Sulakshana Mukherjee mukherji at tifr.res.in
Sat Dec 15 04:23:00 EST 2001


Hello!
Try adding chloramphenicol to the culture to a final conc. of 
1microgram/ml and grow it at 30degC.

with best regards,
Sulakshana

On 13 Dec 2001, shan wrote:

> hi.
> 
> I have a problem regarding the solubility of my protein. This protein has a
> cellulose binding domain tag. the size of the proein is almost 35kDa. This
> protein has huge expression but it is going in the inclusion body in
> insoluble form. I tried to solubilize it by 6M urea it became solubalised
> but when I dialysed against decrease urea gradient it again became
> insoluble.  Some one has the idea how to come out of this problem. any
> suggestion will be appericiated
> 
> shan
> 
> 
> <http://www.biowww.net/forum/read.php?f=1&i=4883&t=4883>
> 

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