Protein purification

Emir REMOVEem at unforgettable.com
Tue Dec 18 02:13:50 EST 2001


What about reverse phase or ion exchange HPLC?
Emir

"Richard Buick" <Richard.Buick at btinternet.com> wrote in message
news:9vfus5$ni8$1 at knossos.btinternet.com...
> Hi
> I wonder if anyone could suggest a method for the separation of active
from
> inactive (mis-folded) protein. Affinity chromatography is not an option
> since the method must be applied to many different proteins. Ion exchange
> and hydrophobic interaction have been tried with not much success. The
> proteins to be separated are post IMAC.
> Thanks
> Rick
>
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