RNA from Monocytes

Angela bookwaam at hotmail.com
Tue Dec 18 21:19:01 EST 2001


I am also having difficultes preparing total RNA from PBMC using a Qiagen
RNeasy kit.  I get roughly 3 ug/million cells for cells stimulated with
LPS.  Is this about the amount of RNA that you are getting?  Is there a
reason you are using the Trizol method vs. RNeasy?


<http://www.biowww.net/forum/read.php?f=3&i=728&t=638>




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