RNA from Monocytes

Angela bookwaam at hotmail.com
Tue Dec 18 21:19:01 EST 2001

I am also having difficultes preparing total RNA from PBMC using a Qiagen
RNeasy kit.  I get roughly 3 ug/million cells for cells stimulated with
LPS.  Is this about the amount of RNA that you are getting?  Is there a
reason you are using the Trizol method vs. RNeasy?


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