Problems with Taq

Damien marsicd at email.uah.edu
Thu Dec 20 19:41:27 EST 2001


Try with 10%DMSO in your reaction mix. It may help.

Good luck,

Damien


"Trond Erik Vee Aune" <teva at online.no> wrote in message
news:3C1DBC95.2000601 at online.no...
> Hi!
>
> I've recently been trying to amplify a gene with Taq and Expand High
> Fidelity. It works fine with Expand but for some reason it doesn't with
> Taq. The reaction set up is identical except for the polymerases and the
> buffers.
>
> It is important that I can do this with Taq since I need its low fidelity.
>
> Do you have any suggestions to what might be wrong? I've tried to
> different batches of Taq with the same negative result.
>
> Sincerely,
> Trond Erik Vee Aune
>
> http://kibt.chembio.ntnu.no/molgen/
> http://www.chembio.ntnu.no/~trondaun/Chaperone-R-US/
>





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