OD and IPTG concentration

Artem Evdokimov AEVDOKIMOZ at cinci.rr.com
Fri Dec 21 22:04:30 EST 2001


"Dominic-Luc Webb molmed" <domweb at mbox.ki.se> wrote in message
news:Pine.GSO.4.43.0112211529240.20977-100000 at mbox.ki.se...

>You will never get that far for the simple reason that
>spectrophotometers peak out at about 3. They will give
>values up to about 4, which are meaninglessly beyond
>the linear range of the spectrophotometer. OD600 are
>absorbance values. Your bugs will already be dying
>at about OD600 of about 0.7.

I am afraid you're not right here. Fermentation easily accomplishes this
kind of culture density.

A.G.E.





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