Smeared Western Samples

Sophie sweiss at icr.ac.uk
Fri Dec 21 08:43:00 EST 2001


Hi everyone,

I thought i'd let you all know that i have been getting better results
recently. I decreased the gel percentage (from 12% to 7%), increased the pH
of the tris from 8.9 to 9.2 and added the tris and SDS to the gel solution
separately instead of using a combined tris/SDS buffer. I also used
ultrapure 10% SDS solution from Gibco instead of making my own SDS solutions
from old SDS powder.

Sophie.


<http://www.biowww.net/forum/read.php?f=6&i=881&t=822>




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