Smeared Western Samples

Sophie sweiss at
Fri Dec 21 08:43:00 EST 2001

Hi everyone,

I thought i'd let you all know that i have been getting better results
recently. I decreased the gel percentage (from 12% to 7%), increased the pH
of the tris from 8.9 to 9.2 and added the tris and SDS to the gel solution
separately instead of using a combined tris/SDS buffer. I also used
ultrapure 10% SDS solution from Gibco instead of making my own SDS solutions
from old SDS powder.



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