Isolation of Cytosol. protein

Tariq Hamid asterhamid at yahoo.com
Mon Dec 31 13:40:29 EST 2001


Hi there
I am also trying to purify a his tagged protein from E.coli. i do get
the protein from the Ni-NTA resin but it isa contaminbated with other
proteins also.I have heard about using glyvero. Can you tell me more
about that.
Tariq
 jhaines at uoguelph.ca (Jeffery Haines) wrote in message news:<9ugplf$j3t$1 at testinfo.cs.uoguelph.ca>...
> I am using Ni-NTA resin to purify a cytosolic protein. I was getting 
> lower  order bands on the SDS-PAGE gels. I tried to purify the protein in 
> the presence of 40% glycerol, and I got rid of the lower order 
> bands but now I am getting some higher order bands. 
> Has anyone experienced a similar problem with their cytosolic protein when 
> they try to purify in the presence of glycerol?   Any suggestions would 
> be greatly appreciated.
> 
> Jaspreet Kaur (jkaur at uoguelph.ca)  
> University of Guelph 
> 
> //Jeff Haines (jhaines at uoguelph.ca)




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