Isolation of Cytosol. protein

Tariq Hamid asterhamid at
Mon Dec 31 13:40:29 EST 2001

Hi there
I am also trying to purify a his tagged protein from E.coli. i do get
the protein from the Ni-NTA resin but it isa contaminbated with other
proteins also.I have heard about using glyvero. Can you tell me more
about that.
 jhaines at (Jeffery Haines) wrote in message news:<9ugplf$j3t$1 at>...
> I am using Ni-NTA resin to purify a cytosolic protein. I was getting 
> lower  order bands on the SDS-PAGE gels. I tried to purify the protein in 
> the presence of 40% glycerol, and I got rid of the lower order 
> bands but now I am getting some higher order bands. 
> Has anyone experienced a similar problem with their cytosolic protein when 
> they try to purify in the presence of glycerol?   Any suggestions would 
> be greatly appreciated.
> Jaspreet Kaur (jkaur at  
> University of Guelph 
> //Jeff Haines (jhaines at

More information about the Methods mailing list