Isolation of Cytosol. protein
asterhamid at yahoo.com
Mon Dec 31 13:40:29 EST 2001
I am also trying to purify a his tagged protein from E.coli. i do get
the protein from the Ni-NTA resin but it isa contaminbated with other
proteins also.I have heard about using glyvero. Can you tell me more
jhaines at uoguelph.ca (Jeffery Haines) wrote in message news:<9ugplf$j3t$1 at testinfo.cs.uoguelph.ca>...
> I am using Ni-NTA resin to purify a cytosolic protein. I was getting
> lower order bands on the SDS-PAGE gels. I tried to purify the protein in
> the presence of 40% glycerol, and I got rid of the lower order
> bands but now I am getting some higher order bands.
> Has anyone experienced a similar problem with their cytosolic protein when
> they try to purify in the presence of glycerol? Any suggestions would
> be greatly appreciated.
> Jaspreet Kaur (jkaur at uoguelph.ca)
> University of Guelph
> //Jeff Haines (jhaines at uoguelph.ca)
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