TUNEL kits?

Cheryl Whittles c.e.whittles at bris.ac.uk
Fri Feb 2 08:03:27 EST 2001

Oncor Appligene do a TUNEL kit. Some false positive staining can occur as well
as false negative (where the DNA is so digested that end-labelling of the DNA
fragments won't occur). Morphological analysis using H & E staining in
combination with IHC for CD45 (lymphocyte marker) is good as another method to
back up results.
Hope this is of some help,

Cheryl Whittles

Dr Cheryl Whittles
Department of Physiology
The New Vet School
Southwell Street
University of Bristol
Bristol BS2 8EJ

"Dr. Klaus Eimert" wrote:

> Dear Netters,
> we want to establish TUNEL staining of apoptotic cells in our lab. Our
> samples are Technovit embedded sections of plant material.
> Does anybody care to share his/her experience with this kind of system? We
> would be interested to know what kind of kits (companies) and staining
> systems people tried and what's their general (and maybe comparetive)
> experience.
> Thanks in advance.
> Regards
> Klaus Eimert
> ---------------------------------------------------------------------------
> Dr. Klaus Eimert
> Dept. of Botany
> State Research Institute Geisenheim     Phone:    + 49 6722 502 469
> Von-Lade-Str. 3                         Fax:      + 49 6722 502 460
> D-65366 Geisenheim                              E-Mail:   eimert at web.de
> Germany           URL: www.mnd.fh-wiesbaden.de/fag/bio/bo/ebostart.html
> ---------------------------------------------------------------------------

More information about the Methods mailing list