palmitoylation

Mark Bowen mbo at slac.stanford.edu
Fri Feb 2 07:43:39 EST 2001


I've tried assaying for palmitoylation by MALDI-TOF and SDS-PAGE.  I see nothing.
I was doing the reaction at room temperature.

How would you recommend I solublize the palmitoyl CoA.  What concentrations are
necessary?

I realize that most people use 3H palmitate to follow labelling.  Is this because
the incorporation is so low as to necessitate this methodology?  If one gets good
stoichiometric incorporation this should not be necessary.

--
Mark Bowen







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