total RNA extraction kit

Sergio sergioal at bbm1.ucm.es
Mon Feb 5 11:02:09 EST 2001



qzt wrote:

> "Sergio" <sergioal at bbm1.ucm.es> ????? news:3A7B061F.605A9A85 at bbm1.ucm.es...
> >
> >
> > clarosa wrote:
> >
> > > Trizol...gibco....simple and highly effective for mRNA
> > >
> > > >
> >
> > I thought you also get the rRNA using trizol...
> > we use Tri-Reagent from SIGMA, which gives fine results on mammalian and
> > bacterial cells.
> >
> > Sergio
> >
> is there any difference in yield or purity between solution based
> and spin column method?

I never tried the column method. The trireagent prepared RNA is ok for northern
and RT-PCR, and we get more than enough for these techniques.
Sergio






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