total RNA extraction kit

clarosa clarosa at biocomp.unl.edu
Mon Feb 5 11:46:57 EST 2001


Trizol gives total RNA.  If you want just messenger, then  a good kit is Qiagen
mRNA mini kit...but there are dozens of other good kits or ways.

Sergio wrote:

> qzt wrote:
>
> > "Sergio" <sergioal at bbm1.ucm.es> ????? news:3A7B061F.605A9A85 at bbm1.ucm.es...
> > >
> > >
> > > clarosa wrote:
> > >
> > > > Trizol...gibco....simple and highly effective for mRNA
> > > >
> > > > >
> > >
> > > I thought you also get the rRNA using trizol...
> > > we use Tri-Reagent from SIGMA, which gives fine results on mammalian and
> > > bacterial cells.
> > >
> > > Sergio
> > >
> > is there any difference in yield or purity between solution based
> > and spin column method?
>
> I never tried the column method. The trireagent prepared RNA is ok for northern
> and RT-PCR, and we get more than enough for these techniques.
> Sergio






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