total RNA extraction kit

Arnoud van Vliet avvliet at knoware.nl
Mon Feb 5 15:50:32 EST 2001


> is there any difference in yield or purity between solution based
> and spin column method?

The column based techniques have a maximum binding capacity, and so if you
load too much, you'll lose a lot of material. Also, the columns can block,
and rRNA might be a bit overrepresented in your sample due to its greater
stability compared to mRNA.

I have used both Qiagens RNeasy, the "old" RNAzol and TRIzol, and I
personally prefer the Trizol/RNAzol method due to its greater flexibility.

hope this helps
Arnoud








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