Shrinking cDNA yield after a few days

R. John Lye rjl6n at virginia.edu
Mon Feb 5 17:48:04 EST 2001


Mathias Droll wrote:

> I am isolation RNA (Chomzynksy) from frozen sections for RT-PCR.
> The cDNA wich i keep in the freezer is very unstable.

At what temperature are you storing the RNA?  We routinely
store RNA at -80 for long periods of time.  RNA stored at
-20 seems tobe degraded fairly quickly.

> The PCR on the same Day of the extraktion works well, but a few day after
> the Extraktion, it  wont work
>
> whats the problem?

I hope that helps,
John








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