Shrinking cDNA yield after a few days
R. John Lye
rjl6n at virginia.edu
Mon Feb 5 17:48:04 EST 2001
Mathias Droll wrote:
> I am isolation RNA (Chomzynksy) from frozen sections for RT-PCR.
> The cDNA wich i keep in the freezer is very unstable.
At what temperature are you storing the RNA? We routinely
store RNA at -80 for long periods of time. RNA stored at
-20 seems tobe degraded fairly quickly.
> The PCR on the same Day of the extraktion works well, but a few day after
> the Extraktion, it wont work
> whats the problem?
I hope that helps,
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