primers problem

Ian A. York iayork at panix.com
Mon Feb 5 20:20:45 EST 2001


In article <kNHf6.1043$Wd4.90848381 at news1.mtl.metronet.ca>,
Tina <anfortin at webnet.qc.ca> wrote:
>
>I diluted the primers in sterile water. The primers were used at 0.25uM,

Primers, like all DNA, degrade in pure water.  Use water buffered (very 
weakly: 10 mM or less) with Tris to a slightly alkaline pH, like 7.5 or 
8.0.

Ian 

-- 
    Ian York   (iayork at panix.com)  <http://www.panix.com/~iayork/>
    "-but as he was a York, I am rather inclined to suppose him a
     very respectable Man." -Jane Austen, The History of England






More information about the Methods mailing list