primers problem

Ian A. York iayork at
Mon Feb 5 20:20:45 EST 2001

In article <kNHf6.1043$Wd4.90848381 at>,
Tina <anfortin at> wrote:
>I diluted the primers in sterile water. The primers were used at 0.25uM,

Primers, like all DNA, degrade in pure water.  Use water buffered (very 
weakly: 10 mM or less) with Tris to a slightly alkaline pH, like 7.5 or 


    Ian York   (iayork at  <>
    "-but as he was a York, I am rather inclined to suppose him a
     very respectable Man." -Jane Austen, The History of England

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