Use of His-binding resin as an affinity column

Patrick Lynch pl229 at medschl.cam.ac.uk
Wed Feb 7 08:46:19 EST 2001


Has anyone ever carried out, or seen a paper, about binding a 6XHis protein
onto a Ni2+ resin and then using this resin as an affinity column?  In my
case, I want to stick a DNA binding protein to the resin and then pass a mix
of DNA molecules through this resin.  Ideally, I would want to elute off the
bound DNA for analysis.

Cheers.

Patrick


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