How much DNA can be electroporated?

Dirk Junghans junghans at ibdm.univ-mrs.fr
Wed Feb 7 09:44:45 EST 2001


Hi,
does anybody know how much Plasmid DNA (ng?) can be electroporated
without loosing efficiency? We are using DH10B cells from Life Techn
(40ul per electroporation). It is important to know because we are
constructing a libary. The company is suggesting 100ng/shot.

Dirk


---






More information about the Methods mailing list