primers problem

Johns053 at mc.duke.edu Johns053 at mc.duke.edu
Wed Feb 7 10:44:34 EST 2001


If you use ammonium hydroxide, you will not be able to kinase your
oligonucleotides.
Stewart




"Dr. Duncan Clark" <Duncan at nospam.demon.co.uk> on 02/07/2001 07:48:44 AM

Please respond to "Dr. Duncan Clark" <Duncan at genesys.demon.co.uk>

To:   methods at hgmp.mrc.ac.uk
cc:
Subject:  Re: primers problem


In article <002a01c09100$1c422ba0$8c01a8c0 at tn.nic.in>, the eminent R.
Jayakumar at School of Biotechnology wrote
>is that true..? Can anyone second this.

Andre Hamel's posts have been suggested to me and that does ring a bell.
The archives are the place to search.

It makes sense. Neat Ammonium hydroxide is used to deprotect and does no
harm so 10mM NaOH should also be OK or maybe better 10mM Amm. Hydroxide
etc.

Duncan
--
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....

Duncan Clark
GeneSys Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk









---






More information about the Methods mailing list