primers problem

Johns053 at Johns053 at
Wed Feb 7 10:44:34 EST 2001

If you use ammonium hydroxide, you will not be able to kinase your

"Dr. Duncan Clark" <Duncan at> on 02/07/2001 07:48:44 AM

Please respond to "Dr. Duncan Clark" <Duncan at>

To:   methods at
Subject:  Re: primers problem

In article <002a01c09100$1c422ba0$8c01a8c0 at>, the eminent R.
Jayakumar at School of Biotechnology wrote
>is that true..? Can anyone second this.

Andre Hamel's posts have been suggested to me and that does ring a bell.
The archives are the place to search.

It makes sense. Neat Ammonium hydroxide is used to deprotect and does no
harm so 10mM NaOH should also be OK or maybe better 10mM Amm. Hydroxide

The problem with being on the cutting edge is that you occasionally get
sliced from time to time....

Duncan Clark
GeneSys Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382


More information about the Methods mailing list