Shrinking cDNA yield after a few days

Victor L. levenson at northwestern.edu
Thu Feb 8 19:30:24 EST 2001


Try storing RNA in formamide - even at 4^C it will be stable for more
than 2 mo. [NB] do not forget to deionize formamide first and then
store it at -20^C in tightly capped tubes.

Cheers,





On Mon, 05 Feb 2001 17:48:04 -0500, "R. John Lye" <rjl6n at virginia.edu>
wrote:

>Mathias Droll wrote:
>
>> I am isolation RNA (Chomzynksy) from frozen sections for RT-PCR.
>> The cDNA wich i keep in the freezer is very unstable.
>
>At what temperature are you storing the RNA?  We routinely
>store RNA at -80 for long periods of time.  RNA stored at
>-20 seems tobe degraded fairly quickly.
>
>> The PCR on the same Day of the extraktion works well, but a few day after
>> the Extraktion, it  wont work
>>
>> whats the problem?
>
>I hope that helps,
>John
>
>
>

Victor Levenson
Director of NUBL
levenson at northwestern.edu
www.biotechlab.nwu.edu






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