Freon alternative

Carl Kirkwood kirkwooc at
Thu Feb 8 19:42:56 EST 2001

Hi all,

We purify virus (rotavirus) using an arklone or freon extraction step, 
before centrifugation.

Would anyone know of alternatives to freon (cannot purchase any more), 
either another more environment-friendly ?? CFC or some other chemical, 
which would allow us to purify the virus.

Dr Carl Kirkwood
Department of Gastroenterology and Clinical Nutrition,
Murdoch Childrens Research Institute,
Royal Children's Hospital,
Parkville, Victoria.

phone: 9345 5069
fax: 9345 6240
email:kirkwooc at


More information about the Methods mailing list