Use of His-binding resin as an affinity column

nieto at porthos.bio.ub.es nieto at porthos.bio.ub.es
Fri Feb 9 01:50:19 EST 2001


This has been done for detection of 
protein-protein interactions. See for instance,
Molecular Micorbiology (1998) 29, 3:859-869

> pl229 at medschl.cam.ac.uk ("Patrick Lynch") wrote:
> :Has anyone ever carried out, or seen a paper, about binding a 6XHis
> protein :onto a Ni2+ resin and then using this resin as an affinity
> column?  In my :case, I want to stick a DNA binding protein to the
> resin and then pass a mix :of DNA molecules through this resin. 
> Ideally, I would want to elute off the :bound DNA for analysis.


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