377 non-denaturing gel

[John Philo]

Susan,

I haven't a clue about your questions, but if you haven't resolved this 
yet my suggestion is to post to query to the Association of 
Biotechnology Resource Facilities list server---lots and lots of 
sequencing folks there. See www.abrf.org for how to post.

John Philo
Alliance Protein Laboratories

Susan S. Newman wrote:

> I need to detect DNA fragments which are up to 1200 base pairs in size and
> are labelled with 6-FAM or 5-FAM on a 377 sequencer using GeneScan.  I must
> use a ROX2500 size standard which requires a non-denaturing gel in order to
> size the fragments correctly.  I am having two problems.  The first is that
> every gel we pour is filled with very tiny bubbles?  We are using 8.1%
> LongRanger with glycerol. We have remade the gel and cleaned the plates
> with no improvement.  I am looking for recommendations for 377 run
> parameters also.  We have already cooked one gel.
> 
> Thanks in advance for any light you are able to shed.
> 
> Susan Newman
> 
> LSU Gene Probes & Expression Systems Laboratory
> "GeneLab"
> Dept. of Pathobiological Sciences
> School of Veterinary Medicine
> Louisiana State University
> Baton Rouge, LA   70803
> 
> Telephone:  225-578-9693  FAX:  225-578-9709
> 
> 
> 
> ---