cloning PCR products

tom chappell tchappell at znet.com
Fri Feb 9 22:07:09 EST 2001


In article <Pine.SGI.3.96.1010115094822.9061733A-100000 at harrier>, "P.J. 
Shaw" <pjs14 at le.ac.uk> wrote:

> On Sun, 14 Jan 2001, Yin Wang wrote:
> 
> > Hi all,
> > I'm looking for an efficient way to clone 1.6 and 2 kb PCR products into
> > pET 20b(+) vector,after trying in vain with cohesive end[EcoR1 ,Hind111]
> > ligation for months.
> > Thanks in advance!!
> > 
> > 
> > 
> Try TOPO cloning in pCRII (Invitrogen). It is foolproof and definitely
> worth the money in terms of time and effort saved. From that, clone into
> your desired pET vector. Should get buckets of fragment with the reuired
> sticky ends.
> 

I work for the company, so this is definitely an ad, but just go to 
www.invitrogen.com. You no longer have to do it as a two step process.






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